Applied Biosystems StepOnePlus

This used Applied Biosystems StepOnePlus Real-Time PCR System is a compact 48-well qPCR instrument designed for labs that need reliable quantitative PCR performance without the footprint of larger 96 or 384-well systems. It supports gene expression analysis, pathogen detection, and genotyping applications, making it a versatile choice for smaller research groups, teaching labs, and clinical testing environments with moderate sample throughput. The StepOnePlus pairs an efficient thermal cycling block with multi-dye optical detection, enabling both single-plex and multiplex assay designs. Its intuitive software walks users through experiment setup, run monitoring, and results analysis, which has made it a popular training and routine-use platform in many molecular biology settings. Despite its smaller well count, it delivers the same core accuracy and repeatability associated with the broader Applied Biosystems real-time PCR line. Sold in used condition, this StepOnePlus system is well suited to labs seeking dependable, space-efficient qPCR capability at a lower cost of entry. It remains compatible with standard TaqMan and SYBR Green assay chemistries, giving new owners a smooth transition into existing testing workflows and validated protocols.