Bio-Rad BioLogic DuoFlow

This used Bio-Rad BioLogic DuoFlow is a modular liquid chromatography workstation built for protein purification and method development in research and process labs. The system integrates pump modules, UV detection, and gradient control under a single software platform, giving users flexible control over flow rate, gradient shape, and fraction collection during a purification run. It is a workhorse platform found in many academic and biotech labs for FPLC style protein separations. The DuoFlow platform is prized for its modularity, allowing labs to configure pumps, detectors, and valves to match specific purification workflows without committing to a fixed hardware footprint. Its chromatography software logs UV traces, conductivity, and pressure in real time, which supports reproducible method development and easy transfer of protocols between operators. Because it was designed for demanding biologics work, the fluid pathways and pump heads are built to handle repeated runs with buffers and solvents common to ion exchange, size exclusion, and affinity chromatography. This unit is sold used and in working condition, having come out of an active laboratory environment where it supported routine protein purification work. It is well suited to labs seeking an economical entry into automated liquid chromatography or looking to add a second purification line without the cost of new capital equipment. Buyers should expect a system ready for reinstallation and requalification within their own lab protocols.